Question

Which technique in RT-PCR is used to detect the presence of the cDNA?

Option 1: Gel Electrophoresis
Option 2: Southern Blotting
Option 3: Northern Blotting
Option 4: Western Blotting

Answer 1

In RT-PCR, the technique used to detect cDNA fragments is gel electrophoresis, followed by Southern blotting to identify specific DNA sequences within a sample.

Step-by-step explanation:

The technique used in RT-PCR to detect the presence of cDNA is gel electrophoresis. Once the DNA fragments have been amplified by RT-PCR, they are separated using gel electrophoresis based on their size. The fragments are then transferred onto a nylon membrane in a procedure known as blotting. Specifically, when DNA is transferred from the gel to a nylon membrane for detection, the technique is called Southern blotting, which is used to identify specific DNA sequences within a sample.