Question

Which of the following factors could contribute to a student obtaining a match with 100% identity, yet having poor scores and E-values?

a) Database size: The match might be against a large database with many similar sequences, leading to lower scores and E-values.

b) Sequence length: If the matched sequence is short, it could result in poor scores and E-values despite having 100% identity.

c) Algorithm parameters: Inappropriate or suboptimal parameters in the alignment algorithm could affect the scoring and E-value calculations.

d) Sequence quality: Poor-quality sequences or sequencing errors may influence the overall alignment scores and E-values.

Answer 1

Factors that could contribute to a student obtaining a match with 100% identity yet having poor scores and E-values include database size, sequence length, algorithm parameters, and sequence quality.

Step-by-step explanation:

Factors that could contribute to a student obtaining a match with 100% identity yet having poor scores and E-values include:

1. Database size: If the match is against a large database with many similar sequences, it can lead to lower scores and E-values.
2. Sequence length: If the matched sequence is short, it could result in poor scores and E-values despite having 100% identity.
3. Algorithm parameters: Inappropriate or suboptimal parameters in the alignment algorithm can affect the scoring and E-value calculations.
4. Sequence quality: Poor-quality sequences or sequencing errors may influence the overall alignment scores and E-values.