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If your BLASTX search has matches to proteins in the database upstream of the first M in your clone, this suggests that what might be the case?

User Rjss
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1 Answer

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Final answer:

Finding protein matches upstream of the first methionine in a BLASTX search suggests alternative or extended open reading frames in the cloned DNA that could result in different protein isoforms.

Step-by-step explanation:

If a BLASTX search shows matches to proteins in the database upstream of the first M (methionine) in your clone, it suggests that there may be additional coding sequences in your cloned DNA that precede the expected start codon.

This could indicate that your cloned sequence contains a different or extended open reading frame (ORF) that encodes a longer or different protein than initially anticipated.

The upstream region could also contain alternative start codons that lead to the production of different protein isoforms.

User Jamiyla
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