Final answer:
cDNA clones might lack the 3' UTR if the synthesis does not proceed to the end of the mRNA molecule, while gDNA clones like BAC and YAC clones will include it due to containing the entire genomic DNA. EST clones may or may not contain the 3' UTR.The right answer is option 1
Step-by-step explanation:
The type of clones that would be missing the 3' UTR in your DNA sequence are cDNA clones. cDNA is synthesized from mRNA, which itself is processed and lacks the intronic regions present in genomic DNA. Since the 3' UTR is part of the mRNA, when reverse transcription occurs to convert mRNA back to DNA to form cDNA, the 3' UTR is included. However, in cases where cDNA synthesis does not proceed to the end of the mRNA molecule, the resulting cDNA might lack the 3' UTR. In contrast, genomic DNA (gDNA) clones, which include BAC and YAC clones, contain the entire genomic DNA, including the 3' UTR. EST clones are similar to cDNA clones, but they are derived from a single sequencing pass from one end and may or may not include the 3' UTR depending on the length of the sequencing read and the position from which the sequencing was initiated.