Question

What are the adaptors used for in Next Generation sequencing?

To attach the DNA fragments to the flow cell surface.
To amplify the DNA fragments.
To barcode the DNA fragments.
All of the above.

Answer 1

Adaptors are used in Next Generation Sequencing to attach DNA fragments to the flow cell surface, amplify the fragments, and barcode them for multiplexing.

Step-by-step explanation:

Adaptors are used in Next Generation Sequencing for multiple purposes:

To attach the DNA fragments to the flow cell surface: Adaptors are added to both ends of the DNA fragments, allowing them to bind to the surface of the flow cell during sequencing.

To amplify the DNA fragments: PCR amplification is carried out using primers designed to anneal to the adapters, creating multiple copies of the DNA fragments.

To barcode the DNA fragments: Unique barcodes or indexes are incorporated into the adaptors, enabling multiple DNA samples to be multiplexed and sequenced simultaneously.