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In genetic research, if the first 30 base pairs of a DNA sequence waveform are deemed unreliable, what is the most appropriate course of action?

A) Discard the entire sequence and start anew.
B) Proceed with the analysis, considering the remaining reliable data.
C) Repeat the sequencing process for the entire DNA strand.
D) Consult additional sources to validate the uncertain segment before continuing the analysis.

1 Answer

6 votes

Final answer:

In genetic research, the best action when faced with unreliable DNA sequence data is to C) repeat the entire sequencing process, a crucial step in ensuring the integrity of the data for accurate analysis, using methods like Sanger's chain termination technique and whole-genome sequencing.

Step-by-step explanation:

In genetic research, when encountering unreliable data, such as the first 30 base pairs of a DNA sequence waveform being deemed unreliable, the most appropriate course of action is to repeat the sequencing process for the entire DNA strand. This is essential as Sanger's method of sequencing, which is a precise method involving the chain termination technique, requires an accurate sequence for further analysis. Since this method sequences one strand at a time and examines one base at a time, any initial errors can lead to unreliable results. Correcting the sequence at the start is critical as the DNA is cut into fragments and sequenced using the chain termination method before being reformed. This ensures the overlapping sequences accurately represent the DNA sequence for further comparison and analysis. Whole-genome sequencing, which determines an entire genome's DNA sequence, is a comprehensive method used in medical sciences to understand genetic diseases. Therefore, ensuring that the sequencing data is accurate from the very beginning is imperative for obtaining reliable and valid results in downstream applications.

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