Final answer:
In Sanger Sequencing, DNA fragments are separated through gel electrophoresis, where they migrate through a gel matrix and create a pattern that can be detected and analyzed. Therefore, the most appropriate correct option is A.
Step-by-step explanation:
The technique used to separate DNA fragments during Sanger Sequencing is gel electrophoresis. This method relies on applying an electric field to a gel matrix, where DNA fragments, charged negatively due to their phosphate backbone, migrate from the negative to the positive end of the gel. The separation occurs based on the size of the DNA fragments, with shorter fragments moving faster and farther than longer ones. This results in a pattern of DNA bands that can be visualized with a fluorescent dye after the separation is complete.
This method is a crucial step in the Sanger sequencing process, which is a chain elongation method developed by a scientist named Frederick Sanger, and it allows one to generate a readout of DNA sequences via an electropherogram.