Question

In Experiment 2 involving siRNA treatment, which control experiments should be included to validate the results shown in Figure 2?

A) Evaluating the protein levels of FASN and mtKAS following siRNA treatment
B) Evaluating the siRNA's specificity for FASN and mtKAS by assessing the cellular levels of unrelated proteins
C) Evaluating the siRNA's specificity by showing that a non-specific siRNA has no effects on the protein levels of FASN and mtKAS
Options:
I and II only
I and III only
II and III only
I, II, and III

Answer 1

To validate results in Figure 2 of Experiment 2 involving siRNA treatment, include control experiments I (evaluating protein levels post-siRNA treatment), II (assessing siRNA specificity on unrelated proteins), and III (demonstrating non-specific siRNA has no impact). So, option I, II, and III are necessary.

Step-by-step explanation:

In Experiment 2 involving siRNA treatment, it's essential to include specific control experiments to validate the results shown in Figure 2. Option A is crucial as it involves evaluating the protein levels of FASN and mtKAS after siRNA treatment, directly assessing the intended impact of the siRNA.

Option B is also important for confirming the siRNA's specificity by assessing its effect on unrelated proteins, ensuring that the observed changes are specific to the targeted genes.

Additionally, Option C is necessary to demonstrate the specificity further; using a non-specific siRNA should not affect the protein levels of FASN and mtKAS. Therefore, all three control experiments (I, II, and III) are vital for comprehensive validation.