Final answer:
Bacon openly defied Berkeley for the first time on June 23, 1676, in Jamestown, when he and his supporters surrounded the statehouse and raised their weapons against the governor.
Step-by-step explanation:
The first time Bacon openly defied Berkeley was on June 23, 1676, in Jamestown. Bacon and his supporters surrounded the statehouse and raised their weapons against the governor. Although Bacon chose not to shoot Berkeley when dared to do so, their showdown resulted in Berkeley fleeing the capital. Bacon's actions in this confrontation prompted the House of Burgesses to award him the commission he wanted and pushed Berkeley to pardon him for his treasonous activities.