Final answer:
UV-vis spectroscopy has many different applications in organic and biological chemistry. One of the most basic of these applications is the use of the Beer-Lambert Law to determine the concentration of a chromophore.
Step-by-step explanation:
UV-vis spectroscopy has many different applications in organic and biological chemistry. One of the most basic of these applications is the use of the Beer-Lambert Law to determine the concentration of a chromophore. The Beer-Lambert Law states that the absorbance of a chromophore at a given wavelength varies linearly with its concentration. This law is commonly used in colorimetry and spectrophotometry to measure the concentration of a substance in a solution.