Question

You have just trypsinized your monolayer cells growing in a T25 flask. Once detached you resuspended all of your cells in media. If you were to subculture some of your cells into a new T25 cells by taking 1.5 mL of cell suspension from a total of 3 mL and transferring it into a new T25 flask containing 8 mL of fresh media, your final dilution would be:

Answer 1

Final dilution is
`(1)/(6.33)`

Step-by-step explanation:

Given

Volume of suspension
`= 3` mL

Volume of subculture
`= 1.5` mL

Volume of fresh media
`= 8` mL

Final dilution

`(1.5)/(8+1.5)`

Final dilution

`= (1.5)/(9.5)\\\\= (15)/(95) \\= (1)/(6.33)`