Question

Why is it critical that disks impregnated with disinfectants be placed as far from one another as possible on the agar plate?

A. If they are too close together, they may enhance one another's effect.
B. If they are too close to one another, it will be hard to interpret the results.
C. If they are too close together, they may antagonize each other's effects.
D. All of the above are correct.

Answer 1

In a disk-diffusion assay, it is important to place disks impregnated with disinfectants far apart on the agar plate to prevent overlapping zones of inhibition and ensure accurate interpretation of results.

Step-by-step explanation:

When conducting a disk-diffusion assay to test the effectiveness of disinfectants, it is critical to place the disks impregnated with disinfectants as far from one another as possible on the agar plate.

This is because if the disks are too close together, they may enhance one another's effect, creating overlapping zones of inhibition that are difficult to interpret. Additionally, if the disks are too close to one another, it can be challenging to accurately measure the diameter of each zone of inhibition. Therefore, the correct answer is B: If they are too close to one another, it will be hard to interpret the results.