Final answer:
The first major threat to Henry's position was the conflict for control over Henry VI's reign by the Duke of York and Duke of Lancaster, beginning his rule in 1422 and leading to the War of the Roses.
Step-by-step explanation:
The first major threat to Henry's position as king was likely the domestic strife caused by the Duke of York and the Duke of Lancaster's conflict to control the young King Henry VI after his ascension to the throne in 1422. This struggle for power set the stage for the War of the Roses, which would plague England until Henry Tudor (Henry VII) emerged victorious in 1485. The tensions and battles for the crown during this period significantly threatened the stability of Henry VI's reign.