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Which type of resolution improves with proper placement of the focal zone at the area of interest?

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Final answer:

Axial resolution in confocal microscopy is improved by properly placing the focal zone at the area of interest, allowing for clearer, three-dimensional imaging, especially in thicker specimens.

Step-by-step explanation:

The type of resolution that improves with proper placement of the focal zone at the area of interest is axial resolution which is enhanced in confocal microscopy. In confocal microscopy, only a single plane of the specimen is in focus, while out-of-focus planes above and below are excluded, resulting in a clearer image.

The placement of the focal zone with precision is pivotal as it ensures that only light from the focal plane is detected by the photomultiplier detector, after passing through a pinhole. This enhances the resolution and quality of the resulting image, which is especially beneficial for analyzing thicker specimens. Image clarity is achieved as the detector scans the specimen, allowing for three-dimensional imaging of structures and live cell processes.

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