Question

A CLS wants to assay for FVIIi antigen in a patient's plasma, so

they set up the following immunoassay procedure:
1. Add patient's' plasma to 96-well plate to bind FVIII to
plate, incubate then wash a

Answer 1

The question addresses a Clinical Laboratory Scientist's methodologies for immunoassay procedures, such as ELISA and hemagglutination assays, to detect specific antigens or antibodies in a patient’s serum, playing a vital role in disease diagnosis and patient immune response monitoring.

Step-by-step explanation:

The question concerns a Clinical Laboratory Scientist (CLS) conducting an immunoassay procedure to assay for Factor VIII (FVIII) antigen in a patient's plasma. The immunoassay techniques generally utilize antibodies or antigens fixed to a solid surface to detect the presence of specific antigens or antibodies in the patient's serum. For instance, the indirect ELISA utilizes antigen from a pathogen attached to microtiter plate wells, capturing antibodies from patient serum to determine a past or current disease. Procedures such as the hemagglutination assay can detect specific types of viruses using a serum that causes agglutination of red blood cells without a microscope. In contrast, the indirect Coombs' test detects antibodies against red blood cell antigens in a patient's serum. These techniques are crucial in diagnosing diseases and monitoring patient's immune response over time.