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Why does DNA flow toward the positive side of the gel chamber?

-none of the answer choices are correct
-Gravity draws the DNA to the positive side.
-DNA has a neutral charge and is attracted by the positive side.
-DNA has a negative charge and is attracted by the positive side.
-DNA has a positive charge and is attracted by the positive side.

User Yic
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1 Answer

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Final answer:

DNA moves toward the positive side in gel electrophoresis because it has a negative charge and is attracted by the positive electrode. The process separates DNA fragments by size, utilizing an agarose gel matrix.

Step-by-step explanation:

DNA has a negative charge and is attracted by the positive side. During gel electrophoresis, DNA fragments are loaded into wells on the side of the gel closest to the negative electrode. An electric current applied across the gel causes the negatively charged DNA to flow toward the positive electrode, through the agarose matrix. This process separates DNA fragments by size, as smaller DNA molecules move faster through the gel compared to larger ones.

User Tannika
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