Question

Order the steps required to sequence a region of DNA using dideoxy sequencing. a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled, dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size denature the double-stranded DNA add a primer. deoxynucleotides, labeled dideoxynucleotides. and DNA polymerase

Answer 1

The steps required to sequence a region of DNA using dideoxy sequencing are denaturing the DNA, adding a primer and nucleotides, extending the primer, terminating the growing chain with a labeled dideoxynucleotide, and performing gel electrophoresis.

Step-by-step explanation:

The steps required to sequence a region of DNA using dideoxy sequencing are:

1. Denature the double-stranded DNA.
2. Add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase.
3. DNA polymerase extends the primer, incorporating deoxynucleotides.
4. A labeled, dideoxynucleotide terminates the growing DNA chain.
5. Perform gel electrophoresis to separate the mixture of DNA fragments by size.

Denaturing the DNA separates the two strands, and the primer binds to the single-stranded template. Then, DNA polymerase extends the primer, incorporating deoxynucleotides. The chain is terminated when a labeled, dideoxynucleotide is added. Gel electrophoresis is performed to separate the DNA fragments by size, allowing the sequence to be read.