Final answer:
In glycolysis, ATP is produced through substrate-level phosphorylation, where a phosphate group is transferred directly to ADP, forming ATP.
Step-by-step explanation:
In glycolysis, ATP molecules are produced by substrate-level phosphorylation. During the energy payoff phase of glycolysis, a phosphate group is removed from an organic molecule and directly transferred to an ADP molecule, hence producing ATP. This mechanism contrasts with oxidative phosphorylation, where ATP is produced by chemiosmosis in the mitochondria during glucose catabolism and requires oxygen.
The ATP molecules produced during the energy payoff phase of glycolysis are formed by substrate-level phosphorylation. In substrate-level phosphorylation, a phosphate group is removed from an organic molecule and is directly transferred to an available ADP molecule, producing ATP. During glycolysis, high-energy phosphate groups from the intermediate molecules are added to ADP to make ATP.