Final answer:
In glycolysis, there is a net gain of two ATP molecules from one molecule of glucose. While four ATPs are produced, two are consumed in the early stages, resulting in the net gain. So, the correct answer is option 2
Step-by-step explanation:
During the biochemical pathway of glycolysis, there is a net gain of two ATP molecules. In this process, a total of four ATP molecules are produced, but two ATP molecules are consumed in the initial steps of glycolysis. This yields a net gain of two ATP molecules from one glucose molecule under anaerobic conditions. Additionally, glycolysis results in the formation of two molecules of NADH and two molecules of pyruvate.
The production of ATP in glycolysis occurs in the steps catalyzed by phosphoglycerate kinase and pyruvate kinase. Despite variations in the transport of NADH into the mitochondria for further oxidative phosphorylation, which can influence the theoretical yield of ATP, the recognized net gain of ATP during glycolysis alone is two.
So, the correct answer is option 2.