Final answer:
Chromosome separation begins at the origin of replication in both eukaryotes and bacteria, with each using specific proteins and enzymes like helicase to initiate the replication process.
Step-by-step explanation:
In eukaryotes, the replication process begins at multiple origins of replication on their chromosomes. In contrast, in bacteria (prokaryotes), chromosome replication begins at the origin of replication, which is located at a specific site on their circular chromosome. The initiation of DNA replication at the origin is recognized and bound by certain proteins, followed by the enzymatic action of helicase, which unwinds the DNA double helix. The resulting structure is bidirectional, with replication forks moving away from the origin. Therefore, chromosome separation begins at the origin of replication in Both eukaryotes and bacteria, although the specific processes and characteristics differ between the two types of organisms.