Final answer:
Gene flow is the critical factor that maintains a species' gene pool and prevents the formation of new species by allowing the exchange of alleles among populations.
Step-by-step explanation:
The most important factor that maintains a species' gene pool and prevents speciation is gene flow. Gene flow refers to the transfer of alleles among populations, which maintains genetic diversity and cohesion within a species. For example, in plants, gene flow can occur through pollination where bees or wind carry pollen to different areas, causing an exchange of genetic material. In animals, gene flow is often the result of migration, such as when young lions leave their pride to join another, bringing new alleles into that population. This regular exchange of alleles is crucial because it reduces genetic differences that could lead to separate species forming over time.