Question

Which of the following is a method for initiating the uptake of a plasmid into a target eukaryotic cell?

A. Electroporation.
B. Pillus.
C. Lambdaphage viral vector.
D. Heat shock in the presence of CaCl2.
E. Both A and D.

Answer 1

The method for initiating the uptake of a plasmid into a target eukaryotic cell is through electroporation. This technique uses a brief electric pulse to create transient pores in the cell membrane, allowing for the introduction of the plasmid DNA.

Step-by-step explanation:

The method for initiating the uptake of a plasmid into a target eukaryotic cell is through electroporation. Electroporation is a technique that uses a brief electric pulse to create transient pores in the cell membrane, allowing for the introduction of the plasmid DNA. This method is commonly used in transfection of cells in cell culture.

Another option listed, heat shock in the presence of CaCl2, is a method used for transforming bacterial cells, not eukaryotic cells. Pili and lambdaphage viral vectors are not specific methods for initiating the uptake of a plasmid into a target eukaryotic cell.