Question

In the Jaffe reaction, creatinine reacts with:

a. Alkaline sulfasalazine solution to produce an orange-yellow complex
b. Potassium iodide to form a reddish-purple complex
c. Sodium nitroferricyanide to yield a reddish-brown color
d. Alkaline picrate solution to yield an orange-red complex

Answer 1

In the Jaffe reaction, creatinine reacts with an alkaline picrate solution to produce an orange-red complex, which is the basis for estimating blood creatinine levels.

Step-by-step explanation:

In the Jaffe reaction, creatinine reacts with an alkaline picrate solution to yield an orange-red complex. The Jaffe reaction is a widely-used method for the biochemical estimation of blood creatinine levels, which is an important indicator of renal (kidney) function. This method involves the formation of a colored complex between creatinine and picric acid in an alkaline medium, which can be measured spectrophotometrically due to its distinct color.