Final answer:
The correct option is b). Na+Cl- had no effect on glucose transport in the simulation because it was designed to transport glucose without the aid of sodium. This suggests that glucose uniport rather than sodium-glucose symport was being simulated.
Step-by-step explanation:
Na⁺Cl⁻ had no effect on glucose transport because sodium is not required for glucose transport in the simulation. Glucose transport in many cells, including kidney and intestinal cells, can occur via a special mechanism known as a sodium-glucose symporter, which uses the energy derived from the movement of sodium down its concentration gradient to transport glucose into the cell, against its own gradient. However, in the context, you're asking about, the simulation must have been designed in such a way that sodium is not required for the glucose transport process, which might be through a GLUT protein allowing for glucose uniport, the specific transport of glucose alone, without the involvement of sodium ions.