Question

This method of using chain-terminating ddNTPs during DNA Polymerase experiments is often called Sanger Sequencing.

A TRUE
B FALSE

Answer 1

The Sanger Sequencing method, or chain termination method, uses fluorescently labeled dideoxynucleotides (ddNTPs) to terminate DNA replication at specific locations, allowing for the determination of the DNA sequence through gel electrophoresis and laser scanning.

Step-by-step explanation:

The method you're referring to for determining the sequence of DNA is indeed the chain termination method, also commonly known as the Sanger Sequencing method.

This technique, developed by Frederick Sanger in 1977, relies on the incorporation of dideoxynucleotides (ddNTPs) during DNA replication. As the ddNTPs lack a hydroxyl group (-OH) at the 3' end of the sugar, they prevent the addition of further nucleotides, effectively terminating the DNA strand elongation at a specific location.

This leads to the generation of DNA fragments of varying lengths. By incorporating ddNTPs labeled with different fluorescent colors, each fragment can be identified and the DNA sequence determined through gel electrophoresis and laser scanning, which reads the resulting fragments as a sequence of colors that correspond to the different bases.