Question

DNA Cloning is done _______________ while PCR is done ________________.

(A) in vitro, in vitro
(B) in vitro, in vivo
(C) in vivo, in vivo
(D) in vivo, in vitro

Answer 1

Both DNA cloning and PCR are performed in vitro, outside a living organism, typically in a lab setting. The correct answer is (A) in vitro, in vitro.

Step-by-step explanation:

DNA cloning is a biotechnological process involving the creation of exact genetic replicas of DNA segments such as genes, and this is done in vitro. Polymerase chain reaction (PCR), on the other hand, is a technique that rapidly amplifies the number of copies of specific DNA sequences in vitro as well, making the amplified DNA available for other analyses. Therefore, the correct answer to the question is (A) in vitro, in vitro.

In vivo refers to processes and reactions occurring within a living organism, while in vitro refers to activities performed outside of a living organism, usually in a lab setting. In the context of biotechnology, both DNA cloning and PCR are typically carried out in vitro to manipulate DNA sequences and produce multiple copies of specific genes or DNA fragments without using living organisms to carry out the process.