Question

Which of the following statements about cDNA libraries are true?

I. cDNA Libraries will lack all introns typically removed by alternative splicing.
II. cDNA Libraries can vary based on the type of cell the RNA was taken from.
III. cDNA Libraries isolated from one cell/cell type can be called Expression Libraries.

(A) I only
(B) I and II only
(C) II and III only
(D) I, II and III

Answer 1

The final answer to the question about cDNA libraries is (D) I, II and III, as all statements are true, with cDNA libraries reflecting the expressed genes in a cell and lacking introns.

Step-by-step explanation:

The correct answer to the question regarding cDNA libraries is (D) I, II and III, meaning that all three statements are true. cDNA libraries are created from mRNA, and thus, lack introns due to the mRNA's prior processing through alternative splicing. These libraries reflect the transcriptome or the subset of genes that are being expressed in a particular cell or cell type at a particular time, which can indeed vary based on the cell from which the RNA was obtained.

Therefore, cDNA libraries can also be referred to as Expression Libraries, as they can isolate and sequence DNA that encodes for proteins being expressed at the moment of mRNA extraction.