Final answer:
The two universal themes likely to be developed in the passage are the importance of relying on one's knowledge and training and that honesty often has positive results. These are developed through the main character's conflict and the notion that literature often contains nuanced themes reflecting human complexity.
Step-by-step explanation:
Part A of the student's question asks which two universal themes are developed in the passage. From the information provided, it can be inferred that the appropriate themes are likely the importance of relying on one's knowledge and training and not second-guessing oneself. This aligns closely with the theme that honesty often has positive results, as being true to one's own knowledge and abilities is a form of honesty with oneself.
For Part B, these themes are developed through the conflict Mark Twain experiences in the excerpt. Initially confident in his ability to run a crossing, Twain begins to second-guess himself as Mr. Bixby introduces additional pressures. This conflict and the character's response to it serve to develop the universal theme that one should trust in their own skills and knowledge to navigate tough situations, and the honest reflection of one's capabilities can lead to positive outcomes.
Furthermore, the provided text suggests that literature often contains multiple themes that cannot be reduced to a single message. The complexities and contradictions of human existence are conveyed through themes that are more nuanced than simple morals or clichés.