Final answer:
The enzyme helicase is responsible for breaking the hydrogen bonds between two strands of DNA to facilitate the formation of the replication fork during DNA replication process.
Step-by-step explanation:
The enzyme that breaks apart the hydrogen bonds between two strands of DNA is helicase. During the process of DNA replication, helicase separates the DNA strands at the origin of replication by breaking the hydrogen bonds holding the two strands together, allowing the replication fork to form. Other enzymes involved in replication include topoisomerase, which alleviates the tension caused by unwinding DNA by breaking and rejoining the phosphate backbone, and DNA polymerase, which adds new nucleotides to form the complementary DNA strand.