Question

Select ALL the statements that are TRUE about this lab.

Note: You must select all the statements to earn credit. Partial answers will not receive partial credit.
Group of answer choices
1) The λmaxλmax for each dye will need to be determined before collecting individual absorbance data.
2) You will need to bring to the lab TWO commercial products to test the concentrations of the dyes.
3) You will need to create ONLY one calibration plot in this lab.
4) Beer's Law will be used to determine the unknown concentration of the dye.
5) We will use titration to determine the dye concentration.

Answer 1

The lab will use Beer's Law to determine the unknown dye concentration after determining the λmax, but may require more than one calibration plot. The number of commercial products to test and the use of titration are not confirmed to be part of the lab based on the given information.

Step-by-step explanation:

To determine the true statements about the lab activity, let's evaluate each statement based on the provided references:

1. Beer's Law will indeed be used to determine the unknown concentration of dye, which is confirmed by its application in calculating RNA and DNA concentrations using UV spectroscopy.
2. Determining the λmax (wavelength of maximum absorbance) for a dye is essential before collecting absorbance data, as seen in the typical usage of UV-Vis spectroscopy and finding Amax.
3. Multiple calibration plots might be required, especially if testing more than one dye with different absorbance properties. The provided information suggests that a single calibration plot might not suffice for all substances.
4. The lab activity mentions neither the number of commercial products to be tested nor the use of titration to determine dye concentration. Therefore, we can't confirm these statements as true based on the information given.