Final answer:
In Jan van Eyck's double portrait of Giovanni Arnolfini and His Bride, the symbolic reference that is not present is the red velvet bed canopy.
Step-by-step explanation:
In Jan van Eyck's double portrait of Giovanni Arnolfini and His Bride, the symbolic references include the single burning candle, the ornate convex mirror, the dog at their feet, and the red velvet bed canopy. However, the symbolic reference that is not present in the painting is the red velvet bed canopy (option D).