Question

Primers are always designed to be complementary to the template DNA strand. Which of these sequences is the complementary sequence to the template sequence 5'-GTGGTCTGATCAACGGTAA-3'?

1) 3'-CACCATGACTAGTTGCCAT-5'
2) 3'-CACCATGACTAGTTGCCAT-3'
3) 3'-GTGGACTGATCAACGGTAA-5'
4) 3'-GTGGACTGATCAACGGTAA-3'

Answer 1

The complementary sequence to the given DNA template 5'-GTGGTCTGATCAACGGTAA-3' is 3'-CACCATGACTAGTTGCCAT-5', adhering to the base-pairing rules where A pairs with T and C pairs with G.

Step-by-step explanation:

The question pertains to understanding how to find the complementary DNA sequence to a given template strand of DNA. Taking into consideration that adenine (A) pairs with thymine (T) and cytosine (C) pairs with guanine (G), we are able to determine which strand is the correct complement to the original 5'-GTGGTCTGATCAACGGTAA-3'. The complementary sequence must be antiparallel and match each nucleotide of the template strand with its corresponding pair. Therefore, the correct answer is sequence 1) 3'-CACCATGACTAGTTGCCAT-5'.