Final answer:
Topoisomerase is the enzyme responsible for relieving supercoiling caused by unwinding of the dsDNA molecule. It breaks and reforms DNA's phosphate backbone ahead of the replication fork to relieve pressure and maintain strand separation.
Step-by-step explanation:
The enzyme responsible for relieving supercoiling caused by unwinding of the dsDNA molecule is topoisomerase. Topoisomerase is responsible for breaking and reforming DNA's phosphate backbone ahead of the replication fork, relieving the pressure generated by the separation of DNA strands. It helps relax the supercoiled DNA and maintains strand separation