Question

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substrate that makes them visible under the light or electron microscope.

1) Immunohistochemistry
2) Immunofluorescence
3) Western blotting
4) ELISA

Answer 1

Immunohistochemistry is the technique that relies on specifically made antibodies conjugated to a substrate to make them visible under a microscope.

Step-by-step explanation:

The technique that depends on the use of antibodies made specifically against a particular protein (antigen) and conjugated to a substrate that makes them visible under the light or electron microscope is Immunohistochemistry. In this technique, the antibodies are used to label specific proteins in tissues, allowing for their localization and identification.