Question

What are the 3 key parts in Rho-independent (intrinsic) termination?

Answer 1

The three key parts in Rho-independent termination are a region rich in C-G nucleotides, the formation of a stable hairpin loop in the mRNA, and a following region of A-T nucleotides that leads to termination of the transcription process.

Step-by-step explanation:

The Rho-independent termination mechanism is a process in prokaryotic gene transcription that involves three key parts:

1. A region rich in C-G nucleotides on the DNA template strand.
2. The formation of a stable hairpin loop in the mRNA that causes RNA polymerase to stall.
3. A subsequent region rich in A-T nucleotides that induces mRNA and RNA polymerase to dissociate from the DNA template.

In contrast, Rho-dependent termination is facilitated by the rho protein, which functions differently from the Rho-independent terminator. The main structure that differentiates between Rho-dependent and Rho-independent termination in prokaryotes is the formation of a hairpin structure in the mRNA during Rho-independent termination.