Question

Which of the following lists the correct order of steps involved in creating a fully transgenic plant?

1) 1. Introduce engineered Ti plasmid into bacteria. 2. Infect plant cells with transformed bacteria. 3. Collect seeds from infected plants. 4. Grow new plants from seed and see which ones are transgenic.
2) 1. Infect plant cells with transformed bacteria. 2. Introduce engineered Ti plasmid into bacteria. 3. Collect seeds from infected plants. 4. Grow new plants from seed, determine which plants are transgenic, then collect seeds from only those plants.
3) 1. Introduce engineered Ti plasmid into bacteria. 2. Infect plant cells with transformed bacteria. 3. Isolate chloroplasts from infected plants. 4. Introduce chloroplasts into cuttings taken from non-infected plants and collect the seeds they produce.
4) 1. Introduce engineered Ti plasmid into bacteria. 2. Infect plant cells with transformed bacteria. 3. Grow plants from cuttings taken from infected plants. 4. Collect seeds produced by cutting-generated plants.

Answer 1

The correct order of steps involved in creating a fully transgenic plant is: 1) Introduce engineered Ti plasmid into bacteria. 2) Infect plant cells with transformed bacteria. 3) Collect seeds from infected plants. 4) Grow new plants from seed and see which ones are transgenic.

Step-by-step explanation:

The correct order of steps involved in creating a fully transgenic plant is:

1. Introduce engineered Ti plasmid into bacteria.
2. Infect plant cells with transformed bacteria.
3. Collect seeds from infected plants.
4. Grow new plants from seed and see which ones are transgenic.

This process involves using the natural transfer of DNA from Agrobacterium tumefaciens to a plant host. The engineered Ti plasmid, which carries the desired DNA fragment, is first introduced into bacteria. The transformed bacteria are then used to infect plant cells. Seeds are collected from the infected plants and new plants are grown from these seeds to determine which ones are transgenic.