Question

You have a drug that blocks the activity of the Proteasome. You treat cells with this drug and you determine the effect of treatment on the abundance of your favorite protein by Western blot analysis. Based on this Western blot you can conclude that:

1) A = proteins larger than YFP are synthesized
2) = drug treatment does not affect YFP in any way
3) = drug treatment causes YFP to be destroyed in the proteasome
4) = drug treatment causes YFP to become ubiquitylated
5) E = drug treatment caused the accumulation of ubiquitylated-YFP

Answer 1

Based on the Western blot analysis, 3) the drug treatment causes YFP to be destroyed in the proteasome.

Step-by-step explanation:

Based on the Western blot analysis, you can conclude that drug treatment causes your favorite protein, YFP, to be destroyed in the proteasome. The proteasome is responsible for the degradation of proteins, and the addition of an ubiquitin group to a protein marks it for degradation. Therefore, the drug that blocks the activity of the proteasome is causing the ubiquitylation and subsequent degradation of YFP.