Final answer:
The statement is true; the Michaelis constant (Km) can indeed be determined using the Lineweaver-Burk plot, which is a double reciprocal plot of the Michaelis-Menten equation.
Step-by-step explanation:
The statement 'The Michaelis constant can be determined using the Lineweaver–Burk plot' is true. The Lineweaver-Burk plot is a double reciprocal graph of the Michaelis-Menten equation that enables more precise calculation of enzyme kinetics, specifically the Michaelis constant (Km) and the maximum reaction velocity (Vmax). By plotting 1/v (where v is the reaction velocity) against 1/[S] (where [S] is the substrate concentration), a straight line is obtained where the intercept on the x-axis corresponds to –(1/Km) and the intercept on the y-axis corresponds to 1/Vmax. This method is beneficial for determining kinetic values since it can help overcome some of the limitations associated with the hyperbolic nature of the Michaelis-Menten plot by providing a linear relationship that is easier to interpret.