Question

Which of the following accurately describes the Lineweaver–Burk plot?

1) It is a double-reciprocal plot.
2) The y intercept is equal to 1/Vmax.
3) The x intercept is -1/Km.
4) It is a linear plot.
5) Its slope is the same as the Eadie–Hofstee plot.

Answer 1

The Lineweaver-Burk plot is a double-reciprocal plot used to determine Km and Vmax in enzyme-catalyzed reactions. It has a linear plot and the y-intercept is equal to 1/Vmax, while the x-intercept is equal to -1/Km.

Step-by-step explanation:

The Lineweaver-Burk plot, also known as the double-reciprocal plot, is a graphical representation of the Lineweaver-Burk equation, which is a linearized form of the Michaelis-Menten equation. It is used to determine the kinetic parameters Km (Michaelis constant) and Vmax (maximum velocity) of enzyme-catalyzed reactions.

The Lineweaver-Burk plot has the y-intercept equal to 1/Vmax and the x-intercept equal to -1/Km. It is a linear plot, and its slope is not the same as the Eadie-Hofstee plot.