Question

A protein that is able to interact with 4 different ligands (A, B, C and D) was analyzed and the Kd value for each ligand is given below, which ligand would have the weakest interaction with this protein?

1) A
2) B
3) C
4) D

Answer 1

To determine which ligand has the weakest interaction with a protein, compare their Kd values; the ligand with the highest Kd value has the weakest interaction.

Step-by-step explanation:

In the context of molecular biology and biochemistry, the Kd value, or dissociation constant, represents the affinity between a protein and its ligand. A higher Kd value indicates a lower affinity, meaning the protein and ligand bind less tightly. Conversely, a lower Kd value denotes a stronger interaction, with tighter binding between the protein and ligand. To determine which of the four ligands (A, B, C or D) has the weakest interaction with the protein, one would compare their respective Kd values provided in the question. The ligand associated with the highest Kd value would have the weakest interaction with the protein.