Final answer:
To prepare a 5% glucose solution, you should mix 450 ml of sterile water with 50 ml of 50% glucose.
Step-by-step explanation:
To prepare a 5% glucose solution, we can use the formula:
%(Volume of Glucose Solution) X (Concentration of Glucose) = %(Volume of Final Solution) X (Concentration of Final Solution)
Let's use the given options to determine the correct preparation:
- Option A: Mixing 250 ml of sterile water with 250 ml of 50% glucose yields a final volume of 500 ml and a concentration of 25% glucose. This is incorrect.
- Option B: Mixing 450 ml of sterile water with 50 ml of 50% glucose yields a final volume of 500 ml and a concentration of 5% glucose. This is the correct preparation.
- Option C: Mixing 500 ml of sterile water with 50 ml of 50% glucose yields a final volume of 550 ml and a concentration of 4.55% glucose. This is incorrect.
- Option D: Mixing 400 ml of sterile water with 100 ml of 50% glucose yields a final volume of 500 ml and a concentration of 10% glucose. This is incorrect.
Therefore, the correct preparation is Option B: Mix 450 ml of sterile water with 50 ml of 50% glucose.