Final answer:
Mendel knew that each F1 generation plant contained two different alleles because both parental phenotypes reappeared in the F2 generation, demonstrating a 3:1 dominant-recessive ratio.
Step-by-step explanation:
In Mendel's pea plants, he knew that each F1 generation contained two alleles encoding different characteristics because both parental phenotypes reappeared in the F2 generation. Therefore, the correct answer is: 2) Both parental phenotypes reappeared in the F2 generation.
When Mendel crossed plants that were homozygous for different traits, all resulting F1 offspring were heterozygous and exhibited the dominant trait. However, not until the F1 plants self-pollinated and produced the F2 generation, did the various combinations of characteristics appear, which included both recessive and dominant traits. This 3:1 dominant-recessive ratio in the F2 generation revealed the presence of two different alleles in the F1 generation: one from each parent.