Final answer:
The observed effect of injecting human surgical GBM cells into the 4) chick embryo brain was the development of tumours.
Step-by-step explanation:
In the article, when human surgical GBM cells were injected into the chick embryo brain, the observed effect was that the chick embryo brain developed tumours. This means that the injection of the GBM cells led to the formation of tumours in the chick embryo brain.