Final answer:
V-type pumps differ from P-type pumps in that they do not use energy from phosphorylation for active transport and are commonly located in plant vacuoles, playing a role in vacuolar acidification and osmoregulation.
Step-by-step explanation:
Unlike P-type pumps, V-type pumps are primarily found in plant vacuoles. Both P-type and V-type pumps are involved in active transport of ions and both transport ions against their concentration gradient. However, V-type pumps do not use energy derived from phosphorylation; instead, they use ATP directly to transport protons and other substances across cellular membranes. P-type pumps, such as the sodium-potassium pump, use ATP to phosphorylate the pump itself, causing a change in its conformation and allowing the movement of ions against their gradient. In summary, while P-type pumps use phosphorylation, V-type pumps do not and are also typically found in plant vacuoles, which helps in acidifying the vacuole and in the process of osmoregulation.