Final answer:
Both GST-tagged and Hia-tagged proteins should be pure and show no presence of tubulin if the purification process is properly conducted, as they are designed to specifically target and isolate the protein of interest.
Step-by-step explanation:
In the context of protein purification, when referring to GST-tagged proteins or Hia-tagged proteins, the goal is typically to achieve a high level of purity whereby only the protein of interest is present without contamination from other cellular proteins, such as tubulin. Assuming the purification process is optimized and performs as intended, both GST-tagged and Hia-tagged proteins should be pure and show no presence of tubulin or other unrelated proteins. So, the correct answer to which tagged proteins are supposed to be pure and show no tubulin is 'C) Both GST and Hia-tagged proteins'. This assumes that the purification protocol specifically targets the tag and removes any non-specifically bound proteins, such as tubulin.