Question

Antimycin is an inhibitor of mitochondrial function and is fairly insoluble in water. It can be dissolved in ethanol at 1 mM and then diluted into water to make a 1 μM solution. How would you make 10 mL of a 1 μM solution of antimycin from the 1 mM stock solution? What would the final concentration of ethanol be?

Add _____ μL of 1mM antimycin solution to ______ mL water. The resulting ethanol concentration would be _____ % v/v.

Answer 1

To prepare 10 mL of a 1 µM antimycin solution, add 10 µL of a 1 mM stock solution to 9.99 mL of water. The final concentration of ethanol in the solution will be 0.1% v/v.

Step-by-step explanation:

To make 10 mL of a 1 µM solution of antimycin from a 1 mM stock solution, you would need to perform a dilution. Since the concentrations are related by a factor of 1000 (1 mM is 1000 µM), you need to add 10 µL of the stock solution to water to reach the desired final concentration. The steps are as follows:

1. Add 10 µL of 1mM antimycin solution to 9.99 mL of water.
2. To find the final ethanol concentration, assume that the stock solution is 100% ethanol. When adding 10 µL to 9.99 mL, the final volume is 10 mL (since 10 µL is negligible compared to 9.99 mL). So, the ethanol concentration is 0.1% v/v (10 µL / 10 mL * 100).