Final answer:
The passage in question does not reference 'Jack and the Beanstalk.' Instead, it highlights the conflict faced by Twain, which escalates tension and emphasizes the theme of relying on knowledge and training, and shows the character's development from confidence to self-doubt.
Step-by-step explanation:
The use of the story of "Jack and the Beanstalk" is not explicitly mentioned in the passage provided. Rather, the conflict in the Mark Twain excerpt focuses on Twain's growing apprehension as he faces the pressure of running a crossing with increased scrutiny by Mr. Bixby. The conflict serves to build tension and interest, leading to the development of the theme, which is to rely on one's knowledge and training. This links into broader concepts such as the crafting of manner, the realization of significance within a narrative, and the impact of theme and conflict on a story's development.
As for the plot and characterization, Twain appears to be the protagonist, while Mr. Bixby and the circumstances create the antagonist role. Over the course of the story, the main character undergoes a change, beginning with confidence and slowly transitioning into self-doubt. This change is crucial to the thematic exploration of the passage.