Final answer:
An increase in temperature during step 4 of the experiment would indicate that a chemical reaction has occurred, as it signifies energy being released.
Step-by-step explanation:
Which observation would indicate that a chemical reaction occurred during the lab investigation?
Among the options given, the one that indicates a chemical reaction is c. the temperature of the vinegar increased during step 4. This is an observable factor that suggests a chemical reaction because all chemical changes involve a transfer of energy. An increase in temperature indicates that energy is being released during a reaction. This is different from a physical change, which may also involve a change in energy, but does not result in a new substance being formed. Observations such as color change, gas production, and formation of a solid precipitate are also indicators of a chemical reaction.