Question

Describe how to prepare 500 ml of a 1X SSC solution from a 20X stock solution.

a) Dilute 25 ml of the 20X solution with 475 ml of distilled water.
b) Dilute 100 ml of the 20X solution with 400 ml of distilled water.
c) Dilute 10 ml of the 20X solution with 490 ml of distilled water.
d) Dilute 50 ml of the 20X solution with 450 ml of distilled water.

Answer 1

To prepare 500 ml of a 1X SSC solution from a 20X stock solution, dilute 10 ml of the 20X solution with 490 ml of distilled water.

Step-by-step explanation:

To prepare 500 ml of a 1X SSC solution from a 20X stock solution, you need to dilute the stock solution with distilled water. The correct option is c) Dilute 10 ml of the 20X solution with 490 ml of distilled water.

1. Measure 10 ml of the 20X stock solution using a measuring pipette.
2. Transfer the measured volume of stock solution to a volumetric flask.
3. Add 490 ml of distilled water to the volumetric flask.
4. Mix the solution well to ensure uniformity.

This will result in a 1X SSC solution with a total volume of 500 ml.