Final answer:
The new boundary line between the U.S. and New Spain was created by the Adams-Onís Treaty of 1819, giving the United States complete control over Florida.
Step-by-step explanation:
The new boundary line between the U.S. and New Spain was created by the Adams-Onís Treaty of 1819. This treaty established the border and gave the United States complete control over Florida. The border was indicated by a red line on the map.
In addition, before the Adams-Onís Treaty, the Spanish accepted the 31st parallel as the border and agreed to the free navigation of the Mississippi River in Pinckney's Treaty of 1795.